作业帮生物化学翻译求助11FIG. 2.HAX-1 can be cleaved by Omi protease in vitro. In vitro translated 35S-labeled HAX-1 protein was incubated at 37 °C without (lane 1) or with 500 ng of recombinant His-Omi134–458 for 2 h (lane 2) and6h(lane 3). The
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生物化学翻译求助11FIG. 2.HAX-1 can be cleaved by Omi protease in vitro. In vitro translated 35S-labeled HAX-1 protein was incubated at 37 °C without (lane 1) or with 500 ng of recombinant His-Omi134–458 for 2 h (lane 2) and6h(lane 3). The
生物化学翻译求助11
FIG. 2.
HAX-1 can be cleaved by Omi protease in vitro. In vitro translated 35S-labeled HAX-1 protein was incubated at 37 °C without (lane 1) or with 500 ng of recombinant His-Omi134–458 for 2 h (lane 2) and6h(lane 3). The reactions were resolved on SDS-PAGE, and the gel was transferred on a PDVF membrane and exposed on X-OMAT-AR film.
HAX-1 Protein Level Decreases during Cell Death—To investigate whether the level of HAX-1 protein is regulated in mammalian cells during cell death, we treated HK-2 cells with various concentrations of cisplatin or H2O2. Fig. 3 shows the protein level of HAX-1 proportionally decreases as the concentration of cisplatin is raised from 30 to 70 µM. HAX-1 protein also decreased in cells treated with increasing concentration of H2O2.
生物化学翻译求助11FIG. 2.HAX-1 can be cleaved by Omi protease in vitro. In vitro translated 35S-labeled HAX-1 protein was incubated at 37 °C without (lane 1) or with 500 ng of recombinant His-Omi134–458 for 2 h (lane 2) and6h(lane 3). The
FIG.2.HAX-1能切割的蛋白酶在体外幸.体外翻译的35S标记HAX-1蛋白孵育在37℃无或500(里1)重组其仪-omi134-458为2小时(里巷2号)and6h(3).反应就解决了电泳、凝胶的转膜及pdvf暴露于X光底片-铁片.HAX-1蛋白质在细胞死亡水平下降至调查是否水平HAX-1蛋白在哺乳动物细胞调节细胞死亡,我们对待HK-2细胞与不同浓度的双氧水或铂.
Fig3显示蛋白质水平HAX-1跌幅的比例提高浓度铂米,由30至70µ HAX-1蛋白质在细胞也减少双氧水浓度增加