A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of \53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Pe

来源:学生作业帮助网 编辑:作业帮 时间:2024/05/15 15:48:47

A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of \53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Pe
A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of \53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Peptide B has a net charge of \31.Which peptide would elute first from a cation-exchange resin?Which would elute first from an anion-exchange resin?

A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of \53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Pe
大概意思是某生物学家想用离子交换色谱分离一个肽,在中性条件下过柱,A的净电荷为3,因为Glu 和 Asp残留物比Arg,Lys,多,b肽净电荷1,问那个先从阳离子交换树脂洗脱下来,那个先从阴离子交换树脂洗脱下来,

一个生物化学家想单独的两个肽通过离子置换色谱法。在流动相的pH值是用在列,一个肽(一)有一个网费3,由于存在更多的谷胺酸和Asp残留fuel赖氨酸,比他的残留物。肽B有净电荷为1。这将首先从一个肽洗提阳离子交换树脂?这将首先从一个阴离子交换树脂洗提?...

全部展开

一个生物化学家想单独的两个肽通过离子置换色谱法。在流动相的pH值是用在列,一个肽(一)有一个网费3,由于存在更多的谷胺酸和Asp残留fuel赖氨酸,比他的残留物。肽B有净电荷为1。这将首先从一个肽洗提阳离子交换树脂?这将首先从一个阴离子交换树脂洗提?

收起